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KMID : 0613820040140010115
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Journal of Life Science
2004 Volume.14 No. 1 p.115 ~ p.120
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Genetic Variation of Korean Lepista nuda
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Kim Seung-Hee
Kim Jong-Bong
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Abstract
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Lepista nuda is a world-wide species which has and international reputation as a excellent edible species. In this study, we investigated the genetic variation and taxonomic relationship of L. nuda and other five Tricholomataceae species were analyzed by random amplied polymorphic DNA (RAPD). 15 kinds of random primers were used. The distance matrix was calculated using UPGMA and phyolgenetic relationship were inferred by neighnor-joining (NJ) method. Various bands of 100bp～1600bp were observed in electrophoretic patterns of RAPD. Nei’s genetic distance was calculated using a total of 228 DNA bands identified, and phylogenetic tree was made. The Nei’s genetic variations of L. nuda, Lepista sordida, Collybia peronata, Collybia confluens, Lyophyllum cinerascens, Laccara laccata were 0～21.3%, 21.2～28.0%, 15.4～23.0%, 14～21.8%, 16.5～34.6%, and 12.4～27.4%, respectively. The consistency index, the retention index and homoplasy index were 0.5217, 0.5769 and 0.5156, respectively. Also, two groups could be made by NJ tree. The genetic distance between L. nuda and C. confluens was closer than that between L. nuda and L. sordida.
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KEYWORD
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Lepista nuda, genetic variation, Random Amplified Polynorphe DNA
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